Bsoft program

bseries

Usage: bseries [options] input.star [input.star]

Aligns series of micrographs using consecutive pairwise cross-correlation.

Actions:

-align 56Align to this reference image (image numbers start at 0).
-filament 5.3,50Box particles along filaments with this interval and mask width (voxels).
-extract 124,100,85Extract particles with these radii (in voxels, one value sets all).
-backgroundCorrect background of extracted particles.
-normalizeNormalize extracted particles.

Parameters:

-verbose 7Verbosity of output.
-datatype uForce writing of a new data type.
-sampling 1.5,1.5,1.5Sampling (A/pixel; a single value can be given).
-resolution 900,300High and low resolution limits for cross-correlation (default 0.1,1000 angstrom).
-origin 110,50,44Origin for tilt axis (default 0,0,0).
-edge 23,12Smooth the edge to a given width, with gaussian decay of a given width.
-fill 127Fill value for particles extending beyond image (default background).
-partbase subvolSet the particle base file name (no extension).
-partpath dir/subdirSet the particle file paths.
-partextension pifSet the particle image file format extension.
-helix 16.2,39.5Helical parameters for filament boxing: helical rise
and angle per asymmetric unit (angstroms and degrees).

Input:

-Mask mask.tifMask file to use for cross-correlation.

Output:

-output file.starOutput parameter file.
-image file.pifOutput multi-image file with aligned images.

Bsoft version 1.5.7-20090605 (64 bit)


Generated by bdoc.pl on Mon Jun 15 11:47:11 2009


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