Bsoft program

bpick

Usage: bpick [options] input.star [input2.star ...]

Picks single particles from 2D (micrograph) or 3D (tomogram) images.

Actions:

-reconstructionsOperate on reconstruction parameters rather than micrographs.
-addAdd micrograph coordinates and particle origins (default not).
-find 120,130Find particles using a ring within these radii (voxels).
-extract 150,110,120Extract particles with these radii (voxels, one sets all).
-backgroundCorrect background of extracted particles.
-normalizeNormalize extracted particles.
-center 23.5,200Center each particle image before extraction, using these resolution limits.

Parameters:

-verbose 7Verbosity of output.
-sampling 1.5,1.5,1.5Sampling (A/pixel; a single value can be given).
-fill 127Fill value for particles extending beyond image (default background).
-base subvolSet the particle base file name (no extension).
-path dir/subdirSet the particle file paths.
-extension pifSet the particle image file format.

Output:

-output file.starOutput parameter file.

Bsoft version 1.5.7-20090605 (64 bit)


Generated by bdoc.pl on Mon Jun 15 11:47:11 2009


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