Bsoft program

bpartsel

Usage: bpartsel [options] input.star [input.star]

Selects single particles for reconstruction and further processing.

Input particle selections are honoured, so that multiple restrictive selections

can be made. To reset for a new selection, use the -all option.

Actions:

-reconstructionsOperate on reconstruction parameters rather than micrographs.
-renumberRenumber particles starting from 1 for each micrograph or reconstruction.
-allReset selection to all particles before other selections.
-consolidateConsolidate selection under one selection number.
-setnumber 3Select given selection number and set others to zero.
-setfom 0.3Set the FOM (must be in the range 0-1).
-group 3022Select particles belonging to this group.
-sets 20,1Generate sets of this size of selected particles,
with a flag not to select across micrograph or reconstruction boundaries.
-series 25,1Select: Particles that are all selected in a series and generating sets.
with a flag not to select across micrograph or reconstruction boundaries.
-alternate 3Select: Assign successive particles to different numbers.
-reselect psi,0.1,0.9Reselect based on a range of values associated with this tag.
-View 0.2,-0.5,0.3,15Select: Within an angular distance from a view: x,y,z,angle.
-sideview 17.8Select: Side views within an angular distance from the equator.
-Euler 2,50,-30,2,0,40Select: Euler angle ranges: psi, theta, phi.
-rank 4,1Select a number of groups from FOM ranking and flag to adjust for defocus.
-fom 0.25,1Select: FOM cutoff and flag to adjust for defocus.
-cv 0.55,1Select: Cross validation cutoff and flag to adjust for defocus.
-top 12.0,1Select: percentage of particles (default 100%), ranked by FOM[index] (default index = 0).
-deviation -2.0,1Select: FOM >= average + factor*standard_deviation, using FOM[index] (default index = 0).
-angle 2.4Select: difference angle between view vectors in focal or tilt series.
-closesttofocusSelect: Particles from micrographs closest to focus.
-furthestfromfocusSelect: Particles from micrographs furthest from focus.
-random 23.6Select: random selection to get the given fraction or percentage.
-bootstrap 527Select: random selection with replacement up to the given number.
-FomfirstSet orientations to first FOM in field-of-view series.
-FombestSet orientations to best FOM in field-of-view series.
-filamentCheck filament directions and sets consistent angles.
-getselectionPrints all the selection numbers.
-show 3Show particle parameters indicated by the selection number.
-FomhistogramShow FOM histogram.

Parameters:

-verbose 7Verbosity of output.
-symmetry C5Point group symmetry (required with the -angle option).

Output:

-output file.starOutput parameter file.
-split 3Split micrograph or reconstruction data blocks into individual files:
Argument: 1-6: number of digits inserted before extension
Argument: "id": micrograph ID's are used as file names.
-Particle views.psPostscript output with particle view and origin distributions.
-Micrograph out.psPostscript output with particle micrograph distributions.
-FOM histogram.psPostscript output with particle FOM distributions.

Bsoft version 1.5.7-20090605 (64 bit)


Generated by bdoc.pl on Mon Jun 15 11:47:11 2009


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