Bsoft program

bmultifit

Usage: bmultifit [options] input.img

Searches orientation space for a set of solutions for the fit of a template within a 3D map.
Modes: Global: Search an asymmetric unit with a fixed angular step size. Symmetry: Search all symmetry-related views using the view from the -View option. Only one image in a multi-image file will be used (see -image option).

Actions:

-mode sym	Mode: global (default) or symmetric.
-subset 25,5	View subset selection from a start offset and with a given size.
-fom 0.35	FOM cutoff to use (default set to avg+std of all FOM values).

Parameters:

-verbose 7	Verbosity of output.
-datatype u	Force writing of a new data type.
-sampling 1.5,1.5,1.5	Sampling (A/pixel; default from input file; a single value can be given).
-origin 110,50,44	Origin for rotations of the template (default taken from template image).
-angles 8.8,2.5,5.2	Step size for alpha, theta and phi, one value sets all (default 45 degrees).
-resolution 15.6,200	Resolution limits for cross-correlation (default 0).
-View 0.3,-0.5,0.8,33	View to initiate search: vector {xyz} and angle (use with -symmetry option).
-symmetry D6	Symmetry: Point group identifier (use with -View option).
-fullasu	Full asymmetric unit (default half).
-image 2	Image number in a multi-image file (default 0=first image).
-bin 3	Binning of input, template and mask by given kernel size,
	output image remains same size as input.

Input:

-Template image.map	Template to search for.
-Mask mask.tif	Mask in reciprocal space to apply during cross-correlation.

Output:

-Postscript plot.ps	Output postscript file with a plot of projection vectors.
-output file.star	Model output file with best orientations.

Bsoft version 1.5.7-20090605 (64 bit)

Generated by bdoc.pl on Mon Jun 15 11:47:11 2009

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